Nanoparticle Based Transfection Reagents
Transfection, which is the process of introducing foreign nucleic acids into host cells, is one of the most powerful molecular biology tools currently in use. As the exogenous DNA or RNA becomes functional in the transfected cells, they exhibit specific behavior otherwise not demonstrated by normal cells. The technology is extensively used in studying gene expression, cellular events, developing drugs, designing knockout systems and conceiving therapeutic strategies.
Scientists typically utilize transfection reagents to help with insertion of DNA or RNA into cancer cell lines. Specific transfection reagents are used for different types of cells in vitro. In vivo nanoparticle-based transfection reagents have been recently developed for delivery of DNA, siRNA and microRNA molecules to xenograft animal models.
Nucleic acid based products, including miRNA, siRNA and gene constructs make shRNA notorious for being difficult to deliver to cells, often being excreted prior to effectiveness. Nucleic acids are vulnerable to nucleases in the blood and the gastrointestinal system. Solutions to this problem are continually under investigation for new polymeric nanoparticles, liposomal formulations and viral vectors. Nanoparticle-based transfection reagents are an emerging product in this area to ensure a high efficiency transfection system.
In spite of the array of transfection reagents already available on the market today, researchers find delivery of external nucleic acids particularly difficult and there is a need for nanoparticle based transfection delivery systems.
Some cells are relatively easy to transfect, whereas others, such as stem cells, suspension cells or primary cells, show poor transfection ability. This is where nanoparticles, each behaving as a single unit, come into the play. Owing to their ultra miniaturized size (ranging from 1 to 100 nanometers), nanoparticles being utilized intensely in today’s research. Nanoparticles hold the promise of applications in the field of biomedicine, especially siRNA transfection for RNAi therapeutics.
Due to the immense therapeutic application of DNA and RNA transfection, especially in cancer treatment, many laboratories are using nanotechnology to come up with nanoparticle based reagents to deliver their payload. Experiments have proven nanoparticles to be efficient vehicles in transporting exogenous nucleic acids into cells.
Silica nanoparticles function by concentrating complexes of transfection reagent molecules and nucleic acid on the cell membrane. Silica nanoparticles in combination with cationic transfection reagents have been shown to enhance transfection efficiency rates.