Polymer-based In Vivo Reagent

Catalog # 5020 - 0.5 mL (10 injections)	$ 425
Catalog # 5021 - 1.5 mL (30 injections)	$ 935

In Vivo Transfection Reagent (Polymer-based)

Plasmid DNA and siRNA in vivo delivery reagent

Modes of administration:

• Systemic intravenous (i.v.) injection
• Direct intratumoral (i.t.) injection

Altogen's In Vivo Transfection Reagent

• Forms siRNA-polymer and plasmid DNA-polymer complexes stable in serum for several hours
• Reagent is Biodegradable Polymer-based
• No detectable toxicity or inflammatory response
• Efficient delivery to the lung, liver, kidney, and spleen via systemic administration
• Efficient siRNA and plasmid DNA transfection via direct subcutaneous tumor injection (various tumor types)
• Functionally tested in mice
• Applicable for siRNA/DNA co-injection
• Transfection protocols are provided with the purchase of the reagent
• Developed and manufactured by Altogen Biosystems

Data

Figure 1. Systemic administration (i.v.) of Polymer-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).


Figure 2. Intratumoral administration (i.t.) of Polymer-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).