HepG2 Transfection Reagent
| Catalog # 2067 - 0.5 mL
|
$ 195 |
 |
| Catalog # 2068 - 1.5 mL
|
$ 387 |
|
 Transfection reagent for HepG2 cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of mRNA, DNA, siRNA and microRNA
- High transfection efficiency
- Low toxicity
- Work in presence of serum
- Compatible with transfection in any plate format
- Reproducible transfection
- Suitable for plasmid DNA/siRNA cotransfection
- Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by Fluorescent microscopy, real-time RT-PCR, Western blot.
Transfection Protocol:
Transfection protocols are provided with the purchase of the reagent.
Shipping information:
US orders are shipped within 1-2 business days via UPS Express. International orders are shipped within 3-5 business days (Europe), and 4-9 business days (Worldwide).
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).
 
|
|
