HCAEC Transfection Reagent
| Catalog # 4104 - 0.5 mL
|
$ 94 |
 |
| Catalog # 4105 - 1.5 mL
|
$ 240 |
|
Transfection reagent for HCAEC cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of DNA, mRNA, siRNA and microRNA
- Efficient for plasmid DNA/siRNA co-transfection
- High cell viability
- Minimal off-target effects
- High knockdown using RNAi
- Cost-effective
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR.
Transfection Protocol:
Transfection protocols are provided with the purchase of the reagent.
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).
