Fibroblast Transfection Reagent
| Catalog # 1755 - 0.5 mL
|
$ 127 |
 |
| Catalog # 1756 - 1.5 mL
|
$ 270 |
|
 Transfection reagent for Fibroblast cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of DNA, mRNA, siRNA and microRNA
- Ideal for primary fibroblast cells
- Low toxicity
- Work in presence of serum
- Reproducible transfection
- Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR.
Transfection Protocol:
Transfection protocols are provided with the purchase of the reagent.
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
 
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