Calu-6 Transfection Reagent
| Catalog # 1727 - 0.5 mL
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$ 79 |
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| Catalog # 1728 - 1.5 mL
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$ 171 |
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 Transfection reagent for Calu-6 cells
- Protocols provided for transfection of DNA, microRNA and siRNA
- High transfection efficiency
- Low toxicity
- Work in presence of serum
- Compatible with transfection in any plate format
- Reproducible transfection
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency: Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent. Shipping information: US orders are shipped within 1-2 business days via UPS Express. International orders are shipped within 3-5 business days (Europe), and 4-9 business days (Worldwide). For maximum stability, store all reagents at +4C upon receipt.

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3). |
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