3LL Transfection Reagent

Catalog # 1701 - 0.5 mL	$ 93
Catalog # 1702 - 1.5 mL	$ 196

Transfection reagent for 3LL cells

• Kit includes Transfection Enhancer reagent and recommended transfection protocols
• Protocols provided for transfection of DNA and siRNA
• Efficient transfection of murine Lewis lung carcinoma cells
• High transfection efficiency
• Work in presence of serum
• Compatible with transfection in any plate format
• Reproducible transfection
• Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:
Reagent exhibits at least 70% transfection efficiency of siRNA delivery. Transfection efficiency was determined by Fluorescent microscopy, real-time RT-PCR, Western blot.

Transfection Protocol:
Transfection protocols are provided with the purchase of the reagent.

Shipping information:
US orders are shipped within 1-2 business days via UPS Express. International orders are shipped within 3-5 business days (Europe), and 4-9 business days (Worldwide).
For maximum stability, store all reagents at +4C upon receipt.

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).