There are a number of technical difficulties associated with inefficient DNA, siRNA or miRNA delivery into cells. These include limited stability of the nucleic acid, inefficient transfer of material across the cell membrane, and poor PK/PD efficacy in vivo. Stable and functional DNA or RNA molecules typically require chemical modifications and carrier molecule encapsulation (liposome-, lipid-, polymer-, etc.) in order to ensure efficient functional effects and minimized off-target effects.
Expert advice is always important for designing specific experiments. Altogen Services offers contract research services to pharmaceutical, biotech and academic institutions worldwide, with a large number of offerings of custom research services. One of the products that Altogen offers is a Nanoparticle In Vivo Reagent. This reagent is administered intratumorally via injection or systemic intravenous injection. The nanoparticle complexes containing a siRNA or plasmid DNA cargo are stable in a serum for 16 hours and can be delivered efficiently to a number of tissues and tumor types. Other in vivo delivery products include lipid-, polymer-, and PEG-Liposome based reagents that are used to protect siRNA from degradation and effectively deliver cargo to the targeted tissue or tumor. An example of a cell line specific transfection reagent is the A549 Transfection Reagent that provides highly efficient delivery of miRNA, siRNA and plasmid DNA into A549 cells with low cytotoxicity.
Altogen Biosystems is a biotechnology company developing cell line specific, pre-optimized transfection reagents for in vitro and in vivo delivery of biomolecules. Molecular Biology labs typically use transfections in order to study gene function and regulation, as well as to perform protein production, biochemical analysis, mutational analysis and gene expression modulation.